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Cost of inoculating seedlings with Pisolithus tinctorius spores
David B. South
Abstract
Although the production of commercial products of vegetative Pt (Pisolithus tinctorius (Pers.) Coker & Couch) inoculums has ceased in North America due to a lack of demand by consumers, the number of products that contain Pt spores has increased. The quality, quantity and price of these products vary considerably. The cost of inoculating 1,000 tree seedlings with Pt basidiospores can vary from $0.45 to more than $30. The cost of treating with Pt spores is lowest when seedlings are inoculated in a container nursery using rates that are less than 0.4 mg per seedling. However, with some products the cost to treat 1,000 bareroot seedlings is greater than $500 when spores are applied in the planting hole. Three decades ago, 1 g of Pt spores could be purchased for $0.13 and now the price of 1 g can exceed $14. Although many research papers provide data on the biological response to inoculating seedlings with spores, few document the cost of inoculation. Commercial products that are marketed toward homeowners containing both ectomycorrhizal and endomycorrhizal spores are more expensive than products that contain only ectomycorrhizal spores. In situations where survival and growth of seedlings are not increased, the benefit/cost ratio will typically be less than one.
Keywords
References
Alvarez, I., & Trappe, J. (1983b). Effects of application rate and cold soaking pretreatment of Pisolithus spores on effectiveness as nursery inoculums on western conifers. Can J Forest Res, 3, 533–537.
Alvarez, I., & Trappe, J. (1983a). Dusting roots of Abies concolor and other conifers with Pisolithus tinctorius spores at outplanting time proves ineffective. Can J Forest Res, 5, 1021–1023.
Amaranthus, M., & Perry, D. (1987). Effect of soil transfer on ectomycorrhiza formation and the growth and survival of conifer seedlings on old, nonreforested clearcuts. Can J Forest Res, 8, 944–951.
Ambriz, E., Báez-Pérez, A., Sánchez-Yáñez, J., Moutoglis, P., & Villegas, J. (2010). Fraxinus-Glomus-Pisolithus symbiosis: plant growth and soil aggregation effects. Pedobiologia, 6, 369–373.
Anonymous. (2014). Trees: from nursery to independence in the landscape -recommendations. 90.
Araujo, G., Sousa, N., Ramos, M., Vega, A., & Castro, P. (2018). Performance of Quercus suber L. at nursery state -application of two bio-inoculants under two distinct environments. Ann Forest Sci, 1, 29.
Báez-Pérez, A., Lindig-Cisneros, R., & Villegas, J. (2017). Survival and growth of nursery inoculated Fraxinus Cram MM, Mexal JG, Souther R (1999) Successful reforestation of South Carolina Sandhills is not influenced by seedling inoculation with Pisolithus tinctorius in the nursery. South J Appl For, 1, 46–52.
Crous, J. (2017). Use of hydrogels in the planting of industrial wood plantations. Southern Forests: A Journal of Forest Science, 3, 197–213.
Davis, A., & Jacobs, D. (2004). First-year survival of northern red oak seedlings planted on former surface coal mines in Indiana. 480–503.
Davis, A., Ross-Davis, A., & Dumroese, K. (2011). Nursery culture impacts cold hardiness in longleaf pine (Pinus palustris) seedlings. Restor Ecol, 6, 717–719.
Demuro, G., Jencks, E., & Hindal, D. (1990). Survival and growth of bigtooth aspen on acidic surface-mine soils as influenced by Pisolithus tinctorius and nitrogen and phosphorus fertilization. American Society of Mining and Reclamation, 573–578.
Dettweiler-Robinson, E., Bakker, J., Evans, J., Newsome, H., Davies, G., Wirth, T., Pyke, D., Easterly, R., Salstrom, D., & Dunwiddie, P. (2013). Outplanting Wyoming big sagebrush following wildfire: stock performance and economics. Rangeland Ecology & Management, 6, 657–666.
Dixon, R., Wright, G., Garrett, H., & Cox, G. (1981). Container-and nursery-grown black oak seedlings inoculated with Pisolithus tinctorius: growth hand ectomycorrhizal development during seedling production period. Can J Forest Res, 3, 487–491.
Dominguez-Núñez, J., Muñóx, D., De La Cruz, A., De Omeñaca, S., & J. (2013). Effects of Pseudomonas fluorescens on the water paramerters of mycorrhizal and non-mycorrhizal seedlings of Pinus halepensis. Agronomy, 3, 571–582.
Duryea, M. (1984). Nursery cultural practices: impacts on seedling quality. 143–164.
Echols, R., Meier, C., Ezell, A., & Mckinley, C. (1990). Dry site survival of bareroot and container seedlings of southern pines from different genetic sources given root dip and ectomycorrhizal treatments. Tree Planters. Notes, 2, 13–21.
Free, G. ;, Manager, Westervelt, Eutaw, A., Garcia-Barreda, S., Molina-Grau, S., & Reyna, S. (2017). Reducing the infectivity and richness of ectomycorhial fungi in a calcareous Quercus ilex forest through soil preparations for truffle plantation establishment: a bioassay study. Fungal Biol-UK, 11, 1137–1143.
González-Ochoa, A., De Las Heras, J., Torres, P., & Sánchez-Gómex, E. (2003). Mycorrhization of Pinus halelpensis Mill. and Pinus pinaster Aiton seedlings in two commercial nurseries. Ann Forest Sci, 1, 43–48.
Grossnickle, S. (2012). Why seedlings survive: importance of plant attributes. New Forest, 711–738.
Grossnickle, S., & South, D. (2017). Seedling quality of southern pines: influence of plant attributes. Tree Planters. Notes, 2, 29–40.
Hamm, P., Campbell, S., & Hansen, E. (1990). Growing healthy seedlings: identification and management of pests in Northwest forest nurseries. Special Publication, 110.
Harrington, J., Wagner, A., & Dreesen, D. (2001). The influence of Pisolithus tinctorius inoculation on greenhouse growth and first-year transplant survival of conifer seedlings. 255–264.
Hatch, A. (1936). The role of mycorrhizae in afforestation. J Forest, 1, 22–29.
Hatchell, G., & Marx, D. (1985). Response of longleaf, sand, and loblolly pines to Pisolithus ectomycorrhizae and fertilizer on sandhills site in South Carolina. Forest Sci, 2, 301–315.
Helm, D., & Carling, D. (1993). Use of soil transfer for reforestation on abandoned mined lands in Alaska. Mycorrhiza, 3, 97–106.
Holusa, J., Peskova, V., Vostra, L., & Pernek, M. (2009). Impact of mycorrhizal inoculation on spruce seedling: comparisons of a 5-year experiment in forests infested by honey fungus. Period Biol, 4, 413–417.
Hua, X., Cordell, C., & Stambaugh, W. (1991). Synthesis of Pisolithus tinctorius ectomycorrhizae and growth responses on some commercially important Chinese tree species. Forest Ecol Manag, 4, 283–292.
Jorgensen, J. (2014). The effects of different concentrations of phosphate on ectomycorrhiza formation by Pisolithus tinctorius. 67.
Khasa, P., Sigler, L., Chakravarty, P., Dancik, B., Erickson, L., & Mccurdy, D. (2001). Effect of fertilization on growth and ectomycorrhizal development of container-grown and bare-root nursery conifer seedlings. New Forest, 3, 179–197.
Kelley, W. (1987). Effect of triadimefon on development of mycorrhizae from natural inoculums in loblolly pine nursery beds. South J Appl For, 1, 49–52.
Kulling, W. (2008). Real case applications of commercial mycorrhiza in the Netherlands. 17–24.
Landis, T. (1993). A practical look at mycorrhizal fungi in nurseries: part two. 12–18.
Lavender, D. (1984). Plant physiology and nursery environment: interactions affecting seedling growth. 133–141.
Leach, G., & Gresham, H. (1983). Early field performance of loblolly pine seedlings with Pisolithus tinctorius ectomycorrhizae on two lower Coastal Plain sites. South J Appl For, 3, 149–153.
Lu, X., Malajczak, N., & Dell, B. (1998). Mycorrhiza formation and growth of Eucalyptus globulus seedlings inoculated with spores of various ectomycorrhizal fungi. Mycorrhiza, 2, 81–86.
Maltz, M., & Treseder, K. (2015). Sources of inocula influence mycorrhizal colonization of plants in restoration projects: a meta-analysis. Restor Ecol, 5, 625–634.
Martin, T., Harris, J., Eaton, G., & Miller, O. (2003). The efficacy of ectomycorrhizal colonization of pin and scarlet oak in nursery production. Journal of Environmental Horticulture, 1, 45–50.
Marx, D. (1976). Synthesis of ectomycorrhizae on loblolly pine seedlings with basidiospores of Pisolithus tinctorius. Forest Sci, 1, 13–20.
Marx, D. (1987). Triadimefon and Pisolithus ectomycorrhizae affect second-year field performance of loblolly pine. Res. Note. SE-349. 6.
Marx, D. (1990). Soil pH and nitrogen influence Pisolithus ectomycorrhizal development and growth of loblolly pine seedlings. Forest Sci, 2, 224–245.
Marx, D., & Barnett, J. (1974). Mycorrhizae and containerized forest tree seedlings. Great Plains Agricultural Council. Publication, 85–92.
Marx, D., & Bell, W. (1985). Formation of Pisolithus ectomycorrhizae on loblolly pine seedlings with spore pellet inoculums applied at different times. Res. Pap. SE-249. 6.
Marx, D., & Bryan, W. (1969). Studies on ectomycorrhizae of pine in an electronically air-filtered, airconditioned, plant-growth room. Can J Bot, 12, 1903–1909.
Marx, D., & Bryan, W. (1975). Growth and ectomycorrhizal development of loblolly pine seedlings in fumigated soil infested with the fungal symbiont Pisolithus tinctorius. Forest Sci, 3, 245–254.
Marx, D., Bryan, W., & Cordell, C. (1976). Growth and ectomycorrhizal development of pine seedlings in nursery soils infested with the fungal symbiont Pisolithus tinctorius. Forest Sci, 1, 91–100.
Marx, D., Bryan, W., & Cordell, C. (1977). Survival and growth of pine seedlings with Pisolithus ectomycorrhae after two years on reforestation sites in North Carolina and Florida. Forest Sci, 3, 363–373.
Marx, D., & Artman, J. (1978). Growth and ectomycorrhizal development of loblolly pine seedlings in nursery soil infested with Pisolithus tinctorius and Thelephora terrestris in Virginia.
(N.d.). 6.
Marx, D., & Cordell, C. (1988). Bed density and Pisolithus ectomycorrhizae affect morphology of loblolly pine seedlings.
Marx, D., & Cordell, C. (1990a). Development of Pisolithus tinctorius ectomycorrhizae on loblolly pine from spores sprayed at different times and rates. Res. Note SE-356. 6.
Marx, D., & Cordell, C. (1990b). Inoculation of fall-and spring-sown longleaf pine seedlings with Pisolithus tinctorius. Res. Note SE-358. 6.
Marx, D., Cordell, C., & France, R. (1986). Effects of triadimefon on growth and ectomycorrhizal development of loblolly and slash pines in nurseries. Phytopathology, 8, 824–831.
Marx, D., Cordell, C., Kenney, D., Mexal, J., Artman, J., Riffle, J., & Molina, R. (1984). Commercial vegetative inoculum of Pisolithus tinctorius and inoculation techniques for development of ectomycorhizae on bare-root tree seedlings. Forest Sci, 3, 25.
Marx, D., Cordell, C., Kormanik, P. ;, Cordell, C., Anderson, R., Hoffard, W., Landis, T., & Smith, R. (1989). a) Mycorrhizae: benefits and practical application in forest tree nurseries.
Marx, D., Cordell, C., Maul, S., & Ruehle, J. (1989). Ectomycorrhizal development on pine by Pisolithus tinctorius in bare-root and container seedling nurseries. New Forest, 1, 57–66.
Marx, D., Jarl, K., Ruehle, J., & Bell, W. (1984). Development of Pisolithus tinctorius ectomycorhizae on pine seedlings using basidiospore-encapsulated seeds. Forest Sci, 4, 897–907.
Marx, D., Marrs, L., & Cordell, C. (2002). Practical use of the mycorrhiza fungal technology in forestry, reclamation, arboriculture, agriculture, and horticulture. Dendrobiology, 1, 27–40.
Marx, D., Mexal, J., & Morris, W. (1979). Inoculation of nursery seedbeds with Pisolithus tinctorius spores mixed with hydromulch increases ectomycorrhizae and growth of loblolly pines. South J Appl For, 4, 175–178.
Marx, D., Morris, W., & Mexal, J. (1978). Growth and ectomycorrhizal development of loblolly pine seedlings in fumigated and nonfumigated nursery soil infested with different fungal symbionts. Forest Sci, 2, 193–203.
Mccomb, A. (1943). Mycorrhizae and phosphorus nutrition of pine seedlings in a prairie soil nursery. Iowa Agricultural Experiment Station Research Bulletin, 582–603.
Mccomb, A., & Griffith, J. (1946). Growth stimulation and phosphorus absorption of mycorrhizal and nonmycorrhizal northern white pine and Douglas fir seedlings in relation to fertilizer treatment. Plant Physiol, 1, 11–17.
Mexal, J. (1980). Aspects of mycorrhizal inoculation in relation to reforestation. New Zealand Journal of Forestry Sci, 1, 208–217.
Mitchell, R., Cox, G., Dixon, R., Garrett, H., & Sander, I. (1984). Inoculation of three species with eleven isolates of ectomycorrhizal fungi. II. Foliar nutrient content and isolate effectiveness. Forest Sci, 3, 563–572.
Molina, R. (1979). Ectomycorrhizal inoculation of containerized Douglas-fir and lodgepole pine seedlings with six isolates of Pisolithus tinctorius. Forest Sci, 4, 585–590.
Pera, J., Parlade, J., & Alvarez, L. (1994). 1, 19–29.
Pilz, D., & Znerold, R. (1986). Comparison of survival enhancement techniques for outplanting on a harsh site in the western Oregon Cacades. Tree Planters. Notes, 4, 24–28.
Pope, P. (1988). Pisolithus tinctorius increases the size of nursery grown red oak seedlings. New Forest, 1, 5–16.
Preve, R., Burger, J., & Kreh, R. (1984). Influence of mine spoil type, fertilizer, and mycorrhizae on pines seeded in greenhouse trays. J Environ Qual, 3, 387–391.
Puttonen, P. (1996). Looking for the “silver bullet” -can one test do it all? New Forest, 1, 52.
Querejeta, J., Roldan, A., Albaladejo, J., & Castillo, V. (1998). The role of mycorrhizae, site preparation, and organic amendment in the afforestation of a semi-arid Mediterranean site with Pinus halepensis. Forest Sci, 2, 203–211.
Repáč, I. (2011). Ectomycorrhizal inoculums and inoculation techniques. Soil Biology, 43–63.
Repáč, I., Balanda, M., Vencurik, J., Kmet, J., Krajmerova, D., & Paule. (2014). Effects of substrate and ectomycorrhizal inoculation on the development of two-years-old container-grown Norway spruce. Picea Abies Karst.) Seedlings. IForest, 4, 487–496.
Repáč, I., Tućeková, A., Sarvašová, I., & Vencurik, J. (2011). Survival and growth of outplanted seedlings of selected tree species on the High Tatra Mts. windthrow area after the first growing season. J Forest Sci, 8, 349–358.
Rincόn, A., Alvarez, I., & Pera, J. (2001). Inoculation of containerized Pinus pinea L. seedlings with seven ectomycorrhizal fungi. Mycorrhiza, 6, 265–271.
Rincόn, A., Alvarez, I., & Pera, J. (2007). Influence of the fertilization method in controlled ectomycorrhizal inoculation of two Mediterranean pines. Ann Forest Sci, 5, 577–583.
Ritchie, G. (1984). Forest Nursery Manual: production of bareroot seedlings. 243–266.
Roldán, A., Querejeta, I., Albaladejo, J., & Castillo, V. (1996). Survival and growth of Pinus halepensis Miller seedlings in a semi-arid environment after forest soil transfer, terracing and organic amendments. Ann Forest Sci, 6, 1099–1112.
Rossi, M., Furigo, A., & Oliveira, V. (2007). Inoculant production of ectomycorrhizal fungi by solid and submerged fermentation. Food Technol Biotech, 3, 277–296.
Ruehle, J. (1980). Inoculation of containerized loblolly pine seedlings with basidiospores of Pisolithus tinctorius. Res. Pap. SE-291, 4.
Ruehle, J., & Marx, D. (1977). Developing ectomycorrhizae on containerized pine seedlings. Res. Pap. SE, 242.
Ruehle, J., Marx, D., Barnett, J., & Pawuk, W. (1981b). Survival and growth of container-grown and bare-root shortleaf pine seedlings with Pisolithus and Thelephora ectomycorrhizae. South J Appl For, 1, 20–24.
Ruehle, J., Marx, D., Barnett, J., & Pawuk, W. (1981a). Development of Pisolithus tinctorius ectomycorrhizae on container-grown pine seedlings as affected by fertility. Forest Sci, 4, 1010–1016.
Sarvaš, M., Pavlenda, P., & Takáčová, E. (2007). Effect of hydrogel application on survival and growth of pine seedlings in reclamations. J Forest Sci, 5, 204–209.
Schooler, J. (2011). Unpublished results hide the decline effect: some effects diminish when tests are repeated. Nature, 437.
Sousa, N., Franco, A., Oliveira, R., & Castro, P. (2012). Ectomycorrhizal fungi as an alternative to the use of chemical fertilizers in nursery production of Pinus pinaster. J Environ Manage, 296-S274.
South, D. (1993). Rationale for growing southern pine seedlings at low seedbed densities. New Forest, 1, 63–92.
South, D. (1998). Needle-clipping longleaf pine and top-pruning loblolly pine in bare-root nurseries. South J Appl For, 4, 235–240.
South, D., & Donald, D. (2002). Effect of nursery conditioning treatments and fall fertilization on survival and early growth of Pinus taeda seedlings in Alabama. U.S.A. Can J Forest Res, 7, 1171–1179.
South, D., Kelley, W., & Chapman, W. (1989). Fall mosaic" of loblolly pine in a forest tree nursery. Tree Planters’ Notes, 1, 19–22.
South, D., & Skinner, M. (1998). Nursery stock and field fertilizer application affect early performance of Pinus radiata on a phosphorus-deficient site in Northland. NZ J Forestry Sci, 3, 361–372.
South, D., Starkey, T., & Enebak, S. (2016). Forest nursery practices in the southern United States. Reforesta, 1, 106–146.
South, D., & Zwolinski, J. (1996). Chemicals used in southern forest nurseries. South J Appl For, 3, 127–135.
Starkey, T., Enebak, S., South, D., & Cross, R. (2012). Particle size and composition of polymer root gels affect loblolly pine seedling survival. Native Plants, 1, 19–26.
Stottlemyer, A., Wang, G., Wells, C., Stottlemyer, D., & Waldrop, T. (2008). Reducing airborne ectomycorrhizal fungi and growing non-mycorrhizal loblolly pine (Pinus taeda L.) seedlings in a greenhouse. Mycorrhiza, 5, 269–275.
Strubbings, J. (1958). Raising and use of large close-rooted transplants for commercial afforestation in southern Rhodesia. South African Forestry Journal, 1, 36–55.
Thapar, H., Paliwal, D. ;, Torres, P., & Honrubia, M. (1982). Inoculation of containerized Pinus halepensis (Miller) seedlings with basidiospores of Pisolithus arhizus (Pers) Rauschert. Rhizopogon Roseolus (Corda) Th M Fr and Suillus Collinitus (Fr) O Kuntze. Annuals of Forest Science, 1, 521–528.
Trofymow, J., & Van Den Driessche, R. (1991). Mycorrhizas. 183–227.
Väre, H. (1990). Effect of soil fertility on root colonization and plant growth of Pinus sylvestris nursery seedlings inoculated with different ectomycorrhizal fungi. Scand J Forest Res, 1, 493–499.
Vosátka, M., Látr, A., & Albrechtová, J. (2008). How to apply mycorrhizal inocula in a large-scale and what outcome can be expected in respect of plant growth and cultivation costs. 323–339.
Wakeley, P. (1954). Planting the southern pines. Agricultural Monograph, 233.
Walker, R. (2002). Fertilization and liming effects on the growth and nutrition of bareroot Jeffrey pine outplanted on an eastern Sierra Nevada surface mine. West J Appl For, 1, 23–30.
Walker, R., & Mclaughlin, S. (1991). Growth and root system development of white oak and loblolly pine as affected by simulated acidic precipitation and ectomycorrhizal inoculation. Forest Ecol Manag, 1, 123–133.
Weissenhorn, I. (2002).
Wilde, S. (1944). Mycorrhizae and silviculture. J Forest, 4, 290–291.
Wilkinson, K. (2009). Nursery Manual for native plants: a guide for tribal nurseries. Agricultural Handbook, 247–261.
Wilkinson, K., Dp ; Wilkinson, J., Landis, K., Haase, T., Daley, D., Dumroese, B., & R. (2014). Tropical Nursery Manual. Agricultural Handbook 732. 253–271.
Wilson, B., & Jacobs, D. (2006). Quality assessment of temperate zone deciduous hardwood seedlings. New Forest, 3, 417–433.
Wiseman, P., Colvin, K., & Wells, C. (2009). Performance of mycorrhizal products marketed for woody landscape plants. Journal of Environmental Horticulture, 1, 41–50.
Whitesell, C., Debell, D., Schubert, T., Strand, R., & Crabb, T. (1992). Short-rotation management of Eucalyptus: guidelines for plantations in Hawaii. Gen, 30.
Wood, G. (1985). Two year survival and growth of loblolly pine seedlings from two Texas seed sources on lignite minesoils. 113.
Young, C. (1981). Open-root nursery techniques and planting methods. South African Forestry Journal, 1, 68–70.
Zak, B., & Bryan, W. (1963). Isolation of fungal symbionts from pine mycorrhizae. Forest Sci, 3, 270–278.
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