The conference on "Reforestation Challenges" took place on 20-22 June 2018 in Belgrade, Serbia, at Faculty of Forestry of the University of Belgrade. This was the fourth meeting on reforestation challenges, after three successful meetings in three previous years (2015 – Belgrade, Serbia; 2016 – Krakow, Poland; 2017 – Session at the IUFRO Congress in Freiburg, Germany). This Conference, like the first one, was organized by Faculty of Forestry of the University of Belgrade, IUFRO (specifically by IUFRO Unit: 3.02.00 - Stand Establishment and Treatment), and Reforesta Scientific and professional Society.

Breaking physical dormancy in some forest seeds is a challenge for scientists and forest managers to obtain an homogeneous germination for larger seed samples. The role played by the seed coat in seed dormancy of six trees with great interest in agroforestry (Robinia pseudoacacia, Leucaena leucocephala, Erythrostemon gilliesii, Styphnolobium japonicum, Acacia dealbata and Brachychiton populneus) was tested by the effects of the pretreatment and its duration on the performance of seed germination, by considering the final germination percentage (FGP) and the mean germination time (MGT). These parameters are estimated at various times of incubation (5, 10 and 15 days) in Petri dishes and stored in darkness at (25 ± 2°C). The pretreatment consists of an immersion of seeds in concentrated sulphuric acid during 30, 60 and 90 minutes. Sowing without pretreatment (control) revealed no germination induction for B. populneus and A. dealbata, except for R. pseudoacacia, L. leucocephala and S. japonica where the germination does not exceed 12% for all the experience duration. Generally, pretreatments were very useful to improve seed germination. The time of immersion into sulphuric acid significantly affected (P < 0.0001) the FGP and the MGT in all studied species. A duration of 30 minutes of soaking was adequate to give a very high rate of germination for L. leucocephala, E. gilliesii, S. japonica and A. dealbata with respective FGP of 100%, 95%, 100% and 100%. However, an extended duration of pretreatment of 60 minutes was necessary for a maximal germination for R. pseudoacacia and B. populneus with FGP of 85% and 100%, respectively. A prolonged duration of 90 minutes of presowing was very fatal for L. leucocephala, A. dealbata and B. populneus. An excellent germinative strength is characterized by a higher FGP and a reduced MGT.

Aiming to produce forest reproductive material at the family level, we tested quality of twenty Fraxinus angustifolia Vahl half-sib lines in the nursery experiment. We measured height (HT) and root collar diameter (DIA) of one-year old seedlings, and we monitored germination rate and mortality during the first growing season. Seeds of 20 half-sib lines originating from Sombor area (north Serbia) were sown in seedbed on autumn 2016. The seed dormancy was not broken in all seeds equally, resulting with un-uniform germination over a two months period. All measured attributes shows a statistically significant differences, indicating a strong effect of genetic control. The highest average and DIA were measured in half-sib line 7 (22.62 cm; 5.79 mm, respectively). The lowest average DIA was measured in half-sib lines 18 (2.85 mm) and 19 (2.94 mm), which can be result of growing density effect (104 seedlings m-2 for line 18 and 126 seedlings m-2 for line 19, compared to 40 seedlings m-2 for line 7). The correlation between DIA and HT is strong and positive (R=0.90). Due to its morphological superiority, half-sib line 7 could be recommended for mass production of Fraxinus angustifolia seedlings at the family level. Keywords: Height; Root Collar Diameter; Seedlings Quality; Narrow leaved ash

This paper provides first report for European white elm potential for biomass production. High density plantation (71,428 seedlings ha-1) was established on spring 2012th near Belgrade from one-year-old seedlings, produced from seed. Weed control and irrigation was practiced only in the first growing season, without fertilization. In a three year rotation, European white elm plants in a high density plantation reach average height of 341.11 cm and average root collar diameter of 31.9 mm, with height increase of about 1 m, diameter increase of about 1 cm per year. At the end of three-years rotation total produced biomass was 90 odt ha-1, or 30 odt ha-1 year-1. Biomass production of European white elm is in range or higher compared to traditionally used species, indicating the need for further research on planting material, plant density and cultural practices.  

Beech is the most important tree species in forests of Serbia. However, despite its significance, the taxonomic status of beech in Serbia is unclear. Morphology of the cupules can be successfully used to distinguish species and within species taxa. In this paper we report results on cupules morphology, measuring the same attributes reported by Mišić (1955): 1) Length of the longest valve of cupule, 2) Width of that valve (at widest point), 3) Distance between base of the longest valve and peduncle, 4) Length of cupule without peduncle, and 5) Length of peduncle. The length of cupule and peduncle length from 12 populations in Serbia are in the range reported for Fagus sylvaticain Serbia and western Eurasia. Results of cluster analysis shows a grouping of populations in two groups:1) the southeast group, and 2) group consist of populations from northwest, east and southeast of Serbia. Populations from this southeast group also consist the group of populations on altitude over 850 m, indicating presence of ecotypes. The exception is population from Stara Planina (1,520 m a.s.l.) which is grouped with populations from altitudes under 850 m. Keywords: European Beech; cupules; variability

Although the production of commercial products of vegetative Pt (Pisolithus tinctorius (Pers.) Coker & Couch) inoculums has ceased in North America due to a lack of demand by consumers, the number of products that contain Pt spores has increased.  The quality, quantity and price of these products vary considerably.  The cost of inoculating 1,000 tree seedlings with Pt basidiospores can vary from $0.45 to more than $30.  The cost of treating with Pt spores is lowest when seedlings are inoculated in a container nursery using rates that are less than 0.4 mg per seedling.  However, with some products the cost to treat 1,000 bareroot seedlings is greater than $500 when spores are applied in the planting hole.  Three decades ago, 1 g of Pt spores could be purchased for $0.13 and now the price of 1 g can exceed $14. Although many research papers provide data on the biological response to inoculating seedlings with spores, few document the cost of inoculation. Commercial products that are marketed toward homeowners containing both ectomycorrhizal and endomycorrhizal spores are more expensive than products that contain only ectomycorrhizal spores.  In situations where survival and growth of seedlings are not increased, the benefit/cost ratio will typically be less than one.

In North America, most tree nursery nutrition publications during the past two decades involved either container-grown stock or stock grown in greenhouses. In contrast, most bareroot nursery fertility trials in North America were published during the last century.  As a result, some bareroot fertilization recommendations have remained the same since 1980 and some practices continue to be based on myths and assumptions. The bareroot nursery industry in the USA might benefit if the next generation of graduate students will consider testing old and new theories about nursery fertilization.  Hopefully, they will discover new facts so that future fertilization regimes will be based on science. This paper provides various fertilizer trials that should be established in bareroot nurseries.