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Online ISSN:
2466-4367

Volume 10 , Issue 2, (2025)

Published:
30.12.2025.

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Reforesta is an Open Access, peer-reviewed online journal published by the Scientific and Professional Society Reforesta.

Reforesta publishes original papers on the fundamental and applied aspects of tree improvement, plant production and quality, and afforestation/reforestation.

Reforesta will consider only original manuscripts that have not been published elsewhere and are not under consideration by another journal.

No article processing charge is applied.

30.06.2020.

Research paper

Effect of temperature and salinity on germination and seedling establishment of Ailanthus altissima (Mill.) Swingle (Simaroubaceae)

The tree of heaven Ailanthus altissima (Mill.) Swingle is a multipurpose tree in forestry. However, it is considered an invasive and dangerous plant for native species, and in particular for national parks, where many studies have recorded their involvement in the disturbance of the already developed floral diversity. Assessing the impact of certain abiotic conditions on this species may identify the expected areas to be colonized by its seed propagation. Germination of tree of heaven were tested for germination at constant temperatures of 25, 30, 40°C, and at room temperature varying from 25-30°C coupled with total darkness. Seeds were sown in Petri dishes (0.8% agar water) for 6 days of incubation. The kinetic of germination was determined according to five closely related parameters viz. final germination percentage (FGP), mean germination time (MGT), coefficient of velocity of germination (CVG), time to 50% germination (T50) and seedling length (SL). For the saline condition, the seeds underwent various NaCl concentrations from 0, 50, 100 or 200 mM. For each treatment, there were four replicates with 50 seeds incubated in a plastic container between two layers of moist sand at 15% of the appropriate treatment and then placed in a culture chamber at 27°C (± 2°C) for 30-day period. The effect of temperature was not significant on the MGT, CVG and T50. However, it was significant (p< 0.0001) on FGP and SL. The maximum germination of 94% was obtained at a temperature of 30°C and the lowest FGP of 40% was obtained at 25°C. For the salinity effect, the FGP of 75% in the control was much higher compared to the seeds treated at 50 mM NaCl with only 17.2% of FGP.  Germination was completely inhibited from 100 mM NaCl. A. altissima can be classified as sensitive to salt stress during seed germination and seedling emergence. The salinity effect then joined the temperature to monitor nature's A. altissima seed propagation.

Abdenour Kheloufi, Lahouaria Mounia Mansouri, Rawnak Zerrouni, Okba Abdelhamid

30.12.2025.

Research paper

Clonal propagation of conifers by somatic embryogenesis (SE) – an introduction to methodology and examples of applications for research and plant production

This article is aimed as a basic introduction to conifer somatic embryogenesis for someone with a general interest in applications of this technique and/or who is interested in starting some practical work in the field. We also present data from an example study on individual trees’ ability to initiate somatic embryogenesis in Norway spruce (Picea abies). A total of 158 trees were each tested for ability and relative competence to initiate somatic embryogenesis from the zygotic embryos by observing the initiation frequency for ten zygotic embryos from each individual tree.  Furthermore, we conducted a case study by following the process for SE plant formation for a selection of 48 cell lines that were monitored through the SE developmental pathway, with data collected on the success rates at different steps. We then evaluated the relative importance of different steps for the outcome of plant formation and yield.

Josefine Björs, Amelia Tudoran, Daniel Gräns, Andreas Helmersson, Adriana Puentes, Elisabeth Wallin, Ulrik Brauner Nilsen, Ulrika Egertsdotter

05.06.2016.

Research paper

The role of tree breeding in reforestation

This article focuses on the creation of seed sources for forest planting or seeding with a special focus on clonal seed orchards supporting planting Norway spruce and Scots pine in Sweden. Supporting long-term breeding and low input breeding is discussed. The focus is not on clonal forestry, although this is discussed. Natural regeneration is not dealt with and provenance choice only briefly. It is not a manual or literature review and focus on my own evaluations, but more detailed reviews can be found in the literature cited. It is intended to contribute some familiarity with many of the relevant genetic aspects on forest plantations.

Dag Lindgren

05.06.2016.

Research paper

The alleviation of reforestation challenges by beneficial soil microorganisms

Surface mining causes major destruction of natural landscapes and ecosystems. The most fertile, surface soil layer is lost permanently, together with vegetation, wildlife, and micro flora. Post-mining areas are characterized with diverse edaphic, topographic, hydrographic conditions, which complicate land restoration. Successful establishment of forest ecosystems on such land depends mostly on selection of tree species. The chosen plants must be capable of tolerating a wide range of acidity, fertility, moisture, and have potential to ameliorate such substrates for more demanding species. But, reforestation of heavily damaged ecosystems, such as post-mining areas, demands a new approach in seedlings production. This new approach takes into account specific requirements of habitat and integrates them into “targeted production of planting material”. A good strategy for successful reforestation of post-mining areas is the input of organic matter (compost, mulch). Also, current knowledge and experiences emphasize the potential of beneficial microorganisms such as, mycorrhizal fungi (MF) and plant growth promoting rhizobacteria (PGPR). The majority of studies that deal with beneficial interactions between trees and microorganisms are focused on the mycorrhiza, while plant growth promoting rhizobacteria are less present in silviculture. In this study, the focus is on the reforestation challenges of two mining basins, Majdanpek and Kolubara and suggests beneficial microorganisms as potential solution. The study presents results of several years’ researches on plant response to the presence of mycorrhizal fungi and PGPR. The substrates used for plant growth were Majdanpek and Kolubara mine deposals. Mycorrhizal seedlings were grown in Majdanpek mine deposal, and at the end of the experiment they had 30% higher biomass in comparison to control (seedlings without mycorrhiza). Seedlings linked with fungi had a higher survival rate. Deposals from Kolubara Mining Basin were used as a substrate for seedlings inoculated with PGPR. In the first experiment, Scots pine and Norway spruce were inoculated with Azotobacter chroococcum, Bacillus megaterium, B. circulans, B. licheniformis, B. pumilus, B. amyloliquefaciens. Inoculation resulted with higher biomass production (Scots pine 43%, Norway spruce 34%). Similar results were obtained in the second experiment where Scots pine and black locust were inoculated with Bacillus licheniformis, Aeromonas hydrophila, Pseudomonas putida and Burkholderia cepacia. Both species had higher biomass (around 20%) in comparison to un-inoculated control. The results confirmed the fact that early establishment and successful growth of vegetation on devastated areas depends on the presence and activity of soil microbes. Microorganisms as a “nature’s solution” pose the potential to alleviate reforestation challenges of anthropogenic devastated landscapes. Their presence and activity is crucial for ecosystem stability. In areas with compromised balance, their introduction is justified action for achieving the goal of long term ecosystem sustainability.

Vera Karličić, Vesna Golubović Ćurguz, Vera Raičević

30.12.2025.

Research paper

Height and diameter performances in afforestation of Taurus cedar and Crimean juniper seedlings: A case study from southern Turkey

The present study was carried out in 16rd year afforestation sampled from southern Turkey of Taurus cedar (Cedrus libani A. Rich) and Crimean juniper (Juniperus excelsa Bieb.). Variation, competition and correlation were examined based on seedling height and stem diameter at base data collected from two samples areas 500 m2 each. Growth performances were different at the areas for the species, while averages of Taurus cedar (223.6 for height and 65.3 mm for stem diameter at base) had higher than Crimean juniper (106 cm and 58.5 mm) in total areas. The results emphasized importance of species selection in afforestation. Significant (p<0.05) differences between areas and species were found according to results of analysis of variance. Positive and significant phenotypic correlation (p<0.05, r>0.52) was estimated between the characteristics in both species and areas. It could be used for tending in afforestation areas.

Yunus Eser

30.12.2025.

Research paper

Different nursery techniques in the production of Quercus crassifolia

Mexico is the country with the highest number of species of the genus Quercus, but there has been little research on their propagation in nurseries. Quercus crassifolia is distributed across more than 50% of the national territory; however, no studies have been conducted to document its morphological growth and plant quality in nurseries for reforestation and forest restoration. Some of the key factors in plant production, principally in a technified system, are the selection of containers, substrates, and fertilizer doses. This study therefore evaluated two container sizes, two mixtures of organic substrates, and two doses of controlled-release fertilizer. After nine months of growth in the nursery, morphometric parameters of the plants were recorded, and quality indices were determined based on these values. The results showed that container size, substrate choice, and fertilization dose all influenced the quality of the plant produced. With both fertilizer doses, the 25:25:50 substrate mixture of fresh pine sawdust, composted pine bark, and moss peat produced plants with the highest values of height, diameter, and shoot, root, and total dry biomass. Considering the factors evaluated, the use of 200 mL containers with the aforementioned substrate mixture and the addition of 7 g L-1 of controlled-release fertilizer will produce plants of Quercus crassifolia suitable quality for use in reforestation.

Rosa Elvira Madrid-Aispuro, José Angel Prieto Ruíz, Arnulfo Aldrete, Silvia Salcido-Ruiz, Alberto Pérez-Luna

05.06.2016.

Research paper

Reforestation challenges in Southeast Europe facing climate change

Projections of the regional climate model for Southeast Europe generally predict an increasing of temperature and a decrease in precipitation, with some local variations. Higher frequency of extreme weather events and increased flooding can also be expected. This climate change will, among other things, result in changes in habitats and species distribution, and a decrease in biodiversity. In most cases, forest ecosystems will be unable to adapt fast enough to keep pace with changes in climate. Extreme weather events and low precipitation during the growing season will cause high mortality of seedlings after planting. New forests will face the whole range of these changes because of the long lifetime of trees. Reforestation programs must take projections of climate change into consideration. In the long term, new guidelines for site-species matching, provenance selection, and genetic diversity need to be adopted. In the short term, site preparation, planting techniques, and post planting protection need to be improved. In addition, seedling quality (morphological, physiological, and genetic) and planting time need to be specific for each site. New site preparation, planting, and post-planting protection methods are useful tools for short term success measured in seedling survival and initial growth. Seedling quality is essential for short and long term success. Different strategies, such as assisted migration and increased genetic diversity of planting material, can provide better chances for long term success measured in growth, fitness, and capability to produce the next, better adapted generation.

Vladan Ivetić, Jovana Devetaković

26.12.2016.

Research paper

Initial height and diameter are equally related to survival and growth of hardwood seedlings in first year after field planting

This study compares the relation of initial height and root collar diameter of bareroot hardwoods seedlings to survival and growth in first year after planting. A total of six species used in Serbian reforestation programs were tested (four native: Fagus sylvatica, Ulmus laevis, Fraxinus excelsior, and Acer pseudoplatanus and two exotic: Robinia pseudoacacia, and Quercus rubra), at 6 sites with a wide range of environmental conditions. Initial seedling height and diameter were equally related to field performance and better in forecasting growth than survival. The relation between seedlings initial morphological attributes and survival was species specific, while for all tested species growth was positively correlated to seedling size at planting. Although large seedlings kept their advantage in size, smaller seedlings grew at a higher rate. Both initial H and D should be considered as equally important in operational programs for hardwoods seedling quality testing.

Vladan Ivetić, Jovana Devetaković, Zoran Maksimović

05.06.2016.

Research paper

Opening Letter

"The best time to plant a tree is twenty years ago. The second best time is now." Chinese proverb There are five million hectares of new forests planted each year, according to Global Forest Resources Assessment (FAO 2015). How successful are we in planting these new forests? What are challenges that practitioners meet today in planting forests? What are challenges that forests established today will face in decades to come? There is a myriad of research results and a body of knowledge large enough to understand principles behind the field performance of planted forests. The environment is dynamic and inputs and outputs continually change, so there is a constant need for new research ranging from the global to microsite scale, and from the ecosystem and species to the population and genotype scale. The journal Reforesta offers a new site for publishing research results, presenting experiences, and bringing forward novel ideas and discussions on reforestation issues. Reforesta editors will strive to combine academic excellence with professional relevance and wish to appeal to both the professional and academic communities. There are a large number of scientific journals, many of which deal with forest ecosystems. So a reasonable question could be: Do we need yet another professional journal? We say yes, because of the following reasons. Reforesta will be a "niche" journal, focusing on research stemming from sourcing seed, to nursery cultural operations, to field planting (e.g., afforestation, reforestation and forest restoration). In addition, Reforesta will cover topics of forest genetics, tree breeding, and stand silviculture. We will encourage the submission of papers that provide interest and value to the international readership dealing with planted forests, such as: 1) novel ideas or approaches to reforestation challenges; 2) connections between plant ecophysiology and seedling field performance; 3) testing of new techniques and products in production of forest reproductive material, and at the planting site; and 4) reviews and discussions on timely and important topics. Establishment of the new journal can be compared to establishing a new forest. Initiation of the Reforesta journal will be supported by the editorial team (i.e. planning and site-species or site-provenance matching). The team will then select appropriate papers from the contributions of willing authors (i.e. selection of planting material, nursery operations, and culling). Then the stage is set: the web-site is operational; the journal is registered and applied for indexing (i.e. site preparation, monitoring and feedback). The inaugural issue is published (i.e. seedlings are planted) and the research community is notified (i.e. post-planting silviculture operations). The parallel between Reforesta and establishing a new forest will continue. The future success of Reforesta will be dependent on the interaction with its contributors and readership. The future size of the Reforesta audience will be dependent on its ability to secure its niche, and to provide services to the professional forestry community, while developing a symbiotic relationship with other forestry journals (i.e. competition and/or facilitation). Performance of Reforesta will be measured by monitoring readership and response levels (i.e. reforestation success) and its lifespan will be defined by its resilience and adaptation potential. Reforesta will follow the open access policy of non-profit journals, with no processing charges applied to any accepted articles and no fees for accessing articles published by Reforesta. This principle ensures that forest research is free and available to forestry professionals in support of a greater global exchange of knowledge. Like planted forests, Reforesta hopes to grow in an open environment with appropriate tending from the editorial team, thereby growing with needs of the professional forestry community. The Reforesta journal has one additional advantage. It is established solely on the enthusiasm of the editorial team. As long as there are enthusiasts recognizing the need for Reforesta, the journal will continue to flourish. The editorial team is here to set the scientific standards and ethical rules for the journal. However, the ultimate direction of Reforesta depends on authors and readership. We are delighted to launch this new journal, a product of a joint venture between enthusiasts ranging from undergraduate students to senior researchers and scientists. Reforesta has one underwriting sponsor - The Scientific and Professional Society Reforesta. We thank them for their support. Under the leadership of Vladan Ivetić, Editor-In-Chief, who initiated the idea for Reforesta, and Steven Grossnickle, Consultant-Editor who plays a supporting role; we have a worthy team. The inaugural issue of the Reforesta journal is in form of Thematic Proceedings resulting from the International Conference on Reforestation Challenges, which took place on 3-6 June 2015 in Belgrade, Serbia. We wish to thank participants who attended the Conference and especially thank the invited authors who contributed to this inaugural issue. We are also grateful to the technical team, made up of excellent young students and researchers. We will continue to develop and fine-tune content to enhance the quality, scope and diversity of Reforesta as the journal grows into an established member of the professional forestry community. 

Vladan Ivetić, Steven C Grossnickle