Establishment of Gmelina arborea plantation in an uncultivated farmland inoculated with arbuscular mycorrhizal fungi and plant growth promoting bacteria
Beneficial microbes are very essential for establishing tree plantations, particularly in wastelands or abandoned lands that make them suitable for cultivation. To establish a plantation in uncultivated farmland Gmelina arborea Roxb. tree seedlings were previously inoculated with the beneficial microbes (Arbuscular mycorrhizal (AM) fungi - Gigaspora albida, Glomus aggregatum), plant growth promoting rhizo bacteria (PGPRs) - Azospirllum brasilense, Bacillus megaterium) in single and combinations. The effect of these beneficial microbes were analysed and it was found that the seedlings inoculated with AM fungi + PGPRs1+ PGPRs2 have improved in growth and biomass (shoot length: 85.3 cm plant-1; root length: 40.2 cm plant-1; number of leaves: 42.3 plant-1; stem girth: 9.5 mm plant-1; shoot dry weight: 8.89 g plant-1; root dry weight: 1.81 g plant-1) than the control and individual microbe inocuations. The tissue nutrients (N: 9.1 mg g-1; P: 9.5 mg g-1; K: 6.1 mg g-1) found higher particularly in AM fungi + PGPRs inoculated seedlings. Thus, improved seedlings were transplanted in an uncultivated farmland and monitored for growth and survival. The seedlings planted in the uncultivated farmland showed significant growth improvement. The single (AM fungi, PGPRs1, PGPRs2) and dual (AM fungi + PGPRs1/PGPRs2) inoculated seedlings of G. arborea showed 75 to 87% survival whereas the combined treatment (AM fungi + PGPRs1 + PGPRs2) showed 96% survival in farmland. These results confirmed that these beneficial microbes significantly contributed to the establishment of G. arborea seedlings in the uncultivated farmland through the transfer of nutrients.
Ajeng, A. A., Abdullah, R., Malek, M. A., Chew, K. W., Ho, Y.-C., Ling, T. C., Lau, B. F., & Show, P. L. (n.d.). The Effects of Biofertilizers on Growth, Soil Fertility, and Nutrients Uptake of Oil Palm (Elaeis Guineensis) under Greenhouse Conditions. Processes, 8(12), 1681. https://doi.org/10.3390/pr8121681
Andriani, Y., Rochima, E., Safitri, R., & Rahayuningsih, S. R. (n.d.). Characterization of Bacillus megaterium and Bacillus mycoides Bacteria as Probiotic Bacteria in Fish and Shrimp Feed. KnE Life Sciences, 2(6), 127. https://doi.org/10.18502/kls.v2i6.1029
Arumugam, K. (n.d.). Afforestation in barren laterite lands with Swietenia macrophylla G. King and plant growth promoting microbes. REFORESTA, 9, 54–65. https://doi.org/10.21750/REFOR.9.07.81
Bagyaraj, D. J. (1992). 19 Vesicular-arbuscular Mycorrhiza: Application in Agriculture. In Methods in Microbiology (pp. 359–373). https://doi.org/10.1016/S0580-9517(08)70102-8
Barua, A., Gupta, S. D., Mridha, M. A. U., & Bhuiyan, M. K. (2010). Effect of arbuscular mycorrhizal fungi on growth of Gmelina arborea in arsenic-contaminated soil. Journal of Forestry Research, 21(4), 423–432. https://doi.org/10.1007/s11676-010-0092-1
Bessadok, K., Navarro-Torre, S., Fterich, A., Caviedes, M. A., Pajuelo, E., Rodríguez-Llorente, I. D., & Mars, M. (2021). Diversity of rhizobia isolated from Tunisian arid soils capable of forming nitrogen-fixing symbiosis with Anthyllis henoniana. Journal of Arid Environments, 188, 104467. https://doi.org/10.1016/j.jaridenv.2021.104467
Burton, J., Chen, C., Xu, Z., & Ghadiri, H. (2010). Soil microbial biomass, activity and community composition in adjacent native and plantation forests of subtropical Australia. Journal of Soils and Sediments, 10(7), 1267–1277. https://doi.org/10.1007/s11368-010-0238-y
Cáceres, E. A. R. (1982). Improved Medium for Isolation of Azospirillum spp. Applied and Environmental Microbiology, 44(4), 990–991. https://doi.org/10.1128/aem.44.4.990-991.1982
Coico, R. (2005). Gram staining. Appendix.3c.
Dickson, A., Leaf, A. L., & Hosner, J. F. (1960). QUALITY APPRAISAL OF WHITE SPRUCE AND WHITE PINE SEEDLING STOCK IN NURSERIES. The Forestry Chronicle, 36(1), 10–13. https://doi.org/10.5558/tfc36010-1
Diouf, D., Duponnois, R., Tidiane Ba, A., Neyra, M., & Lesueur, D. (2005). Symbiosis of Acacia auriculiformis and Acacia mangium with mycorrhizal fungi and Bradyrhizobium spp. improves salt tolerance in greenhouse conditions. Functional Plant Biology, 32(12), 1143–1152. https://doi.org/10.1071/FP04069
Duponnois, R., Plenchette, C., Prin, Y., Ducousso, M., Kisa, M., Bâ, A. M., & Galiana, A. (2007). Use of mycorrhizal inoculation to improve reafforestation process with Australian Acacia in Sahelian ecozones. Ecological Engineering, 29(1), 105–112. https://doi.org/10.1016/j.ecoleng.2006.09.008
Gerdemann, J. W., & Nicolson, T. H. (1963). Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting. Transactions of the British Mycological Society, 46(2), 235–244. https://doi.org/10.1016/S0007-1536(63)80079-0
Giovannini, L., Palla, M., Agnolucci, M., Avio, L., Sbrana, C., Turrini, A., & Giovannetti, M. (n.d.). Arbuscular Mycorrhizal Fungi and Associated Microbiota as Plant Biostimulants: Research Strategies for the Selection of the Best Performing Inocula. Agronomy, 10(1), 106. https://doi.org/10.3390/agronomy10010106
Gunina, A., Smith, A. R., Godbold, D. L., Jones, D. L., & Kuzyakov, Y. (2017). Response of soil microbial community to afforestation with pure and mixed species. Plant and Soil, 412(1–2), 357–368. https://doi.org/10.1007/s11104-016-3073-0
Han, S. O., & New, P. B. (1998). Isolation of Azospirillum spp. from natural soils by immunomagnetic separation. Soil Biology and Biochemistry, 30(8–9), 975–981. https://doi.org/10.1016/S0038-0717(98)00020-0
Hetrick, B. A. D. (1991). Mycorrhizas and root architecture. Experientia, 47(4), 355–362. https://doi.org/10.1007/BF01972077
Jackson, M. L. (1973). Soil chemical analysis (p. 498).
Jensen, M. (2005). Trees commonlycultivated in south east Asia illustrated field guide – RAP publication.
Joner, E. J., & Leyval, C. (2001). Influence of arbuscular mycorrhiza on clover and ryegrass grown together in a soil spiked with polycyclic aromatic hydrocarbons. Mycorrhiza, 10(4), 155–159. https://doi.org/10.1007/s005720000071
Kala, C. P. (2005). Ethnomedicinal botany of the Apatani in the Eastern Himalayan region of India. Journal of Ethnobiology and Ethnomedicine, 1(1). https://doi.org/10.1186/1746-4269-1-11
Karthikeyan, A. (2016). Frankia strains for improving growth, biomass and nitrogen fixation in Casuarina equisetifolia seedlings. J Trop Forest Sci, 28, 235–242.
Karthikeyan, A. (2021). Establishment of Ailanthus tryphysa (Dennst.) Alston inoculated with beneficial microbes in barren laterite rocks. Current Research in Microbial Sciences, 2, 100061. https://doi.org/10.1016/j.crmicr.2021.100061
Karthikeyan, A., & Arunprasad, T. (2021). Growth response of Pterocarpus santalinus seedlings to native microbial symbionts (arbuscular mycorrhizal fungi and Rhizobium aegyptiacum) under nursery conditions. Journal of Forestry Research, 32(1), 225–231. https://doi.org/10.1007/s11676-019-01072-y
Khan, B. M., Hossain, M. K., & Mridha, M. A. U. (2014). Improving Acacia auriculiformis seedlings using microbial inoculant (Beneficial Microorganisms). Journal of Forestry Research, 25(2), 359–364. https://doi.org/10.1007/s11676-013-0421-2
Lauridsen, E. B. (2004). Features of some provenances in an international provenance experiment of Gmelina arborea. New Forests, 28(2–3), 127–145. https://doi.org/10.1023/B:NEFO.0000040941.18907.b0
McEwan, A., Marchi, E., Spinelli, R., & Brink, M. (2020). Past, present and future of industrial plantation forestry and implication on future timber harvesting technology. Journal of Forestry Research, 31(2), 339–351. https://doi.org/10.1007/s11676-019-01019-3
Muthu-Kumar, A., Sandhya, G., & Karthikeyan, A. (2023). EVALUATION OF BIO-FERTILISER (BIO-INOCULANT) CONSORTIA AND THEIR EFFECT ON PLANT GROWTH PERFORMANCE OF SANDALWOOD (SANTALUM ALBUM) SEEDLINGS. JOURNAL OF TROPICAL FOREST SCIENCE, 35(3), 311–321. https://doi.org/10.26525/jtfs2023.35.3.311
Muthukumar, T., & Udaiyan, K. (2006). Growth of Nursery-grown Bamboo Inoculated with Arbuscular Mycorrhizal Fungi and Plant Growth Promoting Rhizobacteria in two Tropical Soil Typeswith and without Fertilizer Application. New Forests, 31(3), 469–485. https://doi.org/10.1007/s11056-005-1380-z
Rajan, S. K., Reddy, B. J. D., & Bagyaraj, D. J. (2000). Screening of arbuscular mycorrhizal fungi for their symbiotic efficiency with Tectona grandis. Forest Ecology and Management, 126(2), 91–95. https://doi.org/10.1016/S0378-1127(99)00089-4
Schenck, N. C., & Perez, Y. (1990). Manual for the identification of VA mycorrhizal fungi (p. 286).
Sharma, M., Delta, A. K., Dhanda, P. S., Kaushik, P., Mohanta, Y. K., Saravanan, M., & Mohanta, T. K. (2022). AMF and PSB applications modulated the biochemical and mineral content of the eggplants. Journal of Basic Microbiology, 62(11), 1371–1378. https://doi.org/10.1002/jobm.202200231
Smith, S. E., & Smith, F. A. (2012). Fresh perspectives on the roles of arbuscular mycorrhizal fungi in plant nutrition and growth. Mycologia, 104(1), 1–13. https://doi.org/10.3852/11-229
Vessey, J. K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, 255(2), 571–586. https://doi.org/10.1023/A:1026037216893
Volk, W. A., & Wheeler, M. (1988). The basic microbiology. New York Harper and Rows Inc, 1, 218.
Wang, Z. (2004). Cultivation and utilization of Gmelina arborea in South Yunnan, China. New Forests, 28(2–3), 201–205. https://doi.org/10.1023/B:NEFO.0000040947.94181.d8
Wardle, D. A., Bardgett, R. D., Klironomos, J. N., Setälä, H., van der Putten, W. H., & Wall, D. H. (2004). Ecological Linkages Between Aboveground and Belowground Biota. Science, 304(5677), 1629–1633. https://doi.org/10.1126/science.1094875
Warrier, R. R., Priya, S. M., & Kalaiselvi, R. (2021). Gmelina arborea– an indigenous timber species of India with high medicinal value: A review on its pharmacology, pharmacognosy and phytochemistry. Journal of Ethnopharmacology, 267, 113593. https://doi.org/10.1016/j.jep.2020.113593
Wollum, A. G. (1982). Cultural Methods for Soil Microorganisms. In Agronomy Monographs (pp. 781–802). https://doi.org/10.2134/agronmonogr9.2.2ed.c37
Yang, N., Ji, L., Salahuddin, Yang, Y., & Yang, L. (2018). The influence of tree species on soil properties and microbial communities following afforestation of abandoned land in northeast China. European Journal of Soil Biology, 85, 73–78. https://doi.org/10.1016/j.ejsobi.2018.01.003
Yooyongwech, S., Phaukinsang, N., Cha-um, S., & Supaibulwatana, K. (2013). Arbuscular mycorrhiza improved growth performance in Macadamia tetraphylla L. grown under water deficit stress involves soluble sugar and proline accumulation. Plant Growth Regulation, 69(3), 285–293. https://doi.org/10.1007/s10725-012-9771-6
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