Beech is the most important tree species in forests of Serbia. However, despite its significance, the taxonomic status of beech in Serbia is unclear. Morphology of the cupules can be successfully used to distinguish species and within species taxa. In this paper we report results on cupules morphology, measuring the same attributes reported by Mišić (1955): 1) Length of the longest valve of cupule, 2) Width of that valve (at widest point), 3) Distance between base of the longest valve and peduncle, 4) Length of cupule without peduncle, and 5) Length of peduncle. The length of cupule and peduncle length from 12 populations in Serbia are in the range reported for Fagus sylvaticain Serbia and western Eurasia. Results of cluster analysis shows a grouping of populations in two groups:1) the southeast group, and 2) group consist of populations from northwest, east and southeast of Serbia. Populations from this southeast group also consist the group of populations on altitude over 850 m, indicating presence of ecotypes. The exception is population from Stara Planina (1,520 m a.s.l.) which is grouped with populations from altitudes under 850 m. Keywords: European Beech; cupules; variability